Abstract
Mycobacterium tuberculosis colonizes, survives, and grows inside macrophages. In vitro macrophage infection models, using both primary macrophages and cell lines, enable the characterization of the pathogen response to macrophage immune pressure and intracellular environmental cues. We describe methods to propagate and infect primary murine bone marrow-derived macrophages and J774 and THP-1 macrophage-like cell lines. We also present methods on the characterization of M. tuberculosis intracellular survival and the preparation of infected macrophages for imaging.
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Acknowledgements
Research in the Abramovitch lab is supported by grants from the Great Lakes Regional Center of Excellence (NIH-NIAID U54AI057153), the NIH-NIAID (R21AI105867), the Michigan Initiative for Innovation and Entrepreneurship (AGR2014-00199), and the Bill and Melinda Gates Foundation (OPP1059227).
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Johnson, B.K., Abramovitch, R.B. (2015). Macrophage Infection Models for Mycobacterium tuberculosis . In: Parish, T., Roberts, D. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 1285. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2450-9_20
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DOI: https://doi.org/10.1007/978-1-4939-2450-9_20
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2449-3
Online ISBN: 978-1-4939-2450-9
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