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Whole-Transcriptome Sequencing for High-Resolution Transcriptomic Analysis in Mycobacterium tuberculosis

  • Andrej Benjak
  • Claudia Sala
  • Ruben C. HartkoornEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1285)

Abstract

RNA-seq uses next-generation sequencing technology to determine the transcription profile of an organism in a quantitative manner. With respect to microarrays, this methodology allows greater resolution, increased dynamic range, and identification of new features such as previously unannotated genes and noncoding RNAs. Here we describe how to extract RNA from mycobacterial cultures, how to prepare libraries for Illumina sequencing, and the bioinformatics analysis of the sequencing data to determine the transcription profile.

Key words

Illumina RNA-seq Transcriptome RNA extraction Library preparation 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Andrej Benjak
    • 1
  • Claudia Sala
    • 1
  • Ruben C. Hartkoorn
    • 1
    Email author
  1. 1.Global Health InstituteÉcole polytechnique fédérale de Lausanne (EPFL)LausanneSwitzerland

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