Aptamer-Modified Magnetic Beads in Affinity Separation of Proteins

Part of the Methods in Molecular Biology book series (MIMB, volume 1286)


Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.

Key words

Aptamer Affinity separation His-tag Protein purification Magnetic beads 



This work was supported by the German Research Foundation DFG.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Institut für Technische ChemieGottfried Wilhelm Leibniz Universität HannoverHannoverGermany

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