Aptamer-Modified Magnetic Beads in Affinity Separation of Proteins
Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.
Key wordsAptamer Affinity separation His-tag Protein purification Magnetic beads
This work was supported by the German Research Foundation DFG.
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