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Tissue-Specific Gene Expression Profiling by Cell Sorting

  • Pui-Leng Ip
  • Kenneth D. BirnbaumEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1284)

Abstract

Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.

Key words

FACS Cell sorting Cell type isolation RNA profiling Plants Quiescent Center 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biology, Center for Genomics and Systems BiologyNew York UniversityNew YorkUSA
  2. 2.Department of Biology, Center for Genomics and Systems BiologyNew York UniversityNew YorkUSA

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