The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1282)

Abstract

Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.

Key words

Tracheal organ culture Ciliostasis Respiratory virus Viral assay 

References

  1. 1.
    McGee ZA, Woods ML (1987) Use of organ cultures in microbiological research. Ann Rev Microbiol 41:291–300CrossRefGoogle Scholar
  2. 2.
    Tyrell DAJ, Bynoe ML (1965) Cultivation of novel type of common-cold virus in organ cultures. Br Med J 5448:1467–1470CrossRefGoogle Scholar
  3. 3.
    Cummiskey JF, Hallum JV, Skinner MS et al (1973) Persistent Newcastle disease virus infection in embryonic chicken tracheal organ cultures. Infect Immun 8:657–664PubMedCentralPubMedGoogle Scholar
  4. 4.
    Higgins PG, Ellis EM (1972) The isolation of influenza viruses. J Clin Pathol 25:521–524CrossRefPubMedCentralPubMedGoogle Scholar
  5. 5.
    Cherry JD, Taylor-Robinson D (1970) Large quantity production of chicken embryo organ culture and use in virus and mycoplasma studies. Appl Microbiol 19:658–682PubMedCentralPubMedGoogle Scholar
  6. 6.
    Hodgson T, Casais R, Dove B et al (2004) Recombinant infectious bronchitis coronavirus Baudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity. J Virol 78:13804–13811CrossRefPubMedCentralPubMedGoogle Scholar
  7. 7.
    Cook JKA, Darbyshire JH, Peters RW (1976) The use of chicken tracheal organ cultures for the isolation and assay of infectious bronchitis virus. Arch Virol 50:109–118CrossRefPubMedGoogle Scholar
  8. 8.
    Yacida S, Aoyam S, Takahashi N et al (1978) Plastic multiwell plates to assay avian infectious bronchitis virus in organ cultures of chicken embryo trachea. J Clin Microbiol 8:380–387Google Scholar
  9. 9.
    Battacharjee PS, Naylor CJ, Jones RC (1994) A simple method for immunofluorescence staining of tracheal organ cultures for the rapid identification of infectious bronchitis virus. Avian Pathol 23:471–480CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.The Pirbright InstituteBerkshireUK

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