The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration

  • Ruth M. Hennion
Part of the Methods in Molecular Biology book series (MIMB, volume 1282)


Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.

Key words

Tracheal organ culture Ciliostasis Respiratory virus Viral assay 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.The Pirbright InstituteBerkshireUK

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