Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.
Tracheal organ culture Ciliostasis Respiratory virus Viral assay
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McGee ZA, Woods ML (1987) Use of organ cultures in microbiological research. Ann Rev Microbiol 41:291–300CrossRefGoogle Scholar
Tyrell DAJ, Bynoe ML (1965) Cultivation of novel type of common-cold virus in organ cultures. Br Med J 5448:1467–1470CrossRefGoogle Scholar
Cummiskey JF, Hallum JV, Skinner MS et al (1973) Persistent Newcastle disease virus infection in embryonic chicken tracheal organ cultures. Infect Immun 8:657–664PubMedCentralPubMedGoogle Scholar
Cherry JD, Taylor-Robinson D (1970) Large quantity production of chicken embryo organ culture and use in virus and mycoplasma studies. Appl Microbiol 19:658–682PubMedCentralPubMedGoogle Scholar
Hodgson T, Casais R, Dove B et al (2004) Recombinant infectious bronchitis coronavirus Baudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity. J Virol 78:13804–13811CrossRefPubMedCentralPubMedGoogle Scholar
Cook JKA, Darbyshire JH, Peters RW (1976) The use of chicken tracheal organ cultures for the isolation and assay of infectious bronchitis virus. Arch Virol 50:109–118CrossRefPubMedGoogle Scholar
Yacida S, Aoyam S, Takahashi N et al (1978) Plastic multiwell plates to assay avian infectious bronchitis virus in organ cultures of chicken embryo trachea. J Clin Microbiol 8:380–387Google Scholar
Battacharjee PS, Naylor CJ, Jones RC (1994) A simple method for immunofluorescence staining of tracheal organ cultures for the rapid identification of infectious bronchitis virus. Avian Pathol 23:471–480CrossRefGoogle Scholar