Abstract
Quartz crystal microbalance with dissipation monitoring (QCM-D) is a useful technique for observing the adsorption of molecules onto a protein-functionalized surface in real time. This technique is based on relating changes in the frequency of a piezoelectric sensor chip, onto which molecules are adsorbing, to changes in mass using the Sauerbrey equation. Here, we outline the cleaning, preparation, and analysis involved in a typical QCM-D experiment, from which one can obtain mass adsorption and kinetic binding information.
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References
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Acknowledgements
We would like to acknowledge the Pomona College Chemistry Department and Pomona College Summer Undergraduate Research Program for their continual support.
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Heller, G.T., Mercer-Smith, A.R., Johal, M.S. (2015). Quartz Microbalance Technology for Probing Biomolecular Interactions. In: Meyerkord, C., Fu, H. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 1278. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2425-7_9
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DOI: https://doi.org/10.1007/978-1-4939-2425-7_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2424-0
Online ISBN: 978-1-4939-2425-7
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