Quantitative Analysis of Protein-Protein Interactions

  • Ziad M. Eletr
  • Keith D. Wilkinson
Part of the Methods in Molecular Biology book series (MIMB, volume 1278)


Numerous authors, including contributors to this volume, have described methods to detect protein-protein interactions. Many of these approaches are now accessible to the inexperienced investigator thanks to core facilities and/or affordable instrumentation. This chapter discusses some common design considerations that are necessary to obtain valid measurements, as well as the assumptions and analytical methods that are relevant to the quantitation of these interactions.

Key words

Ligand binding Protein-protein interaction Fluorescence Binding equations Binding equilibria 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of BiochemistryEmory University School of MedicineAtlantaUSA

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