Quantitative Analysis of Protein-Protein Interactions
Numerous authors, including contributors to this volume, have described methods to detect protein-protein interactions. Many of these approaches are now accessible to the inexperienced investigator thanks to core facilities and/or affordable instrumentation. This chapter discusses some common design considerations that are necessary to obtain valid measurements, as well as the assumptions and analytical methods that are relevant to the quantitation of these interactions.
Key wordsLigand binding Protein-protein interaction Fluorescence Binding equations Binding equilibria
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