Abstract
Biolayer Interferometry (BLI) is a powerful technique that enables direct measurement of biomolecular interactions in real time without the need for labeled reagents. Here we describe the analysis of a high-affinity binding interaction between a monoclonal antibody and purified antigen using BLI. A simple Dip-and-Read™ format in which biosensors are dipped into microplate wells containing purified or complex samples provides a highly parallel, user-friendly technique to study molecular interactions. A rapid rise in publications citing the use of BLI technology in a wide range of applications, from biopharmaceutical discovery to infectious diseases monitoring, suggests broad utility of this technology in the life sciences.
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Kumaraswamy, S., Tobias, R. (2015). Label-Free Kinetic Analysis of an Antibody–Antigen Interaction Using Biolayer Interferometry. In: Meyerkord, C., Fu, H. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 1278. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2425-7_10
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DOI: https://doi.org/10.1007/978-1-4939-2425-7_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2424-0
Online ISBN: 978-1-4939-2425-7
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