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Identification of Genes Essential for Leptospirosis

  • Thanatchaporn Bartpho
  • Gerald L. MurrayEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1279)

Abstract

The development of methods for the construction of defined mutants of pathogenic Leptospira has been a breakthrough in the study of leptospiral virulence. These methods have allowed the identification of genes essential for infection in animal models. This chapter describes methods for random transposon mutagenesis of pathogenic leptospires, identification of transposon insertion sites using direct sequencing from genomic DNA and a nested PCR utilizing degenerate oligonucleotides, and methods for testing mutant attenuation in the hamster model of infection.

Key words

Leptospira Leptospirosis Himar1 Transposon mutagenesis 

Notes

Acknowledgements

Ben Adler is thanked for reading the manuscript and helpful suggestions.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of MicrobiologyMonash UniversityClaytonAustralia
  2. 2.Department of MicrobiologyMonash UniversityClaytonAustralia

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