Abstract
High-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy is a nondestructive technique that is used to obtain the metabolite profile of a tissue sample. This method requires minimal sample preparation. However, it is important to handle the sample with care and keep it frozen during preparation to minimize degradation. Here, we describe a typical protocol for HR-MAS analysis of intact tissue. We also include examples of typical pulse sequence programs and quantification methods that are used today.
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Acknowledgments
The authors thank senior engineer Trygve Andreassen for useful consultation regarding MRS acquisition procedures.
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Giskeødegård, G.F., Cao, M.D., Bathen, T.F. (2015). High-Resolution Magic-Angle-Spinning NMR Spectroscopy of Intact Tissue. In: Bjerrum, J. (eds) Metabonomics. Methods in Molecular Biology, vol 1277. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2377-9_4
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DOI: https://doi.org/10.1007/978-1-4939-2377-9_4
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