High-Resolution Magic-Angle-Spinning NMR Spectroscopy of Intact Tissue

  • Guro F. Giskeødegård
  • Maria D. Cao
  • Tone F. Bathen
Part of the Methods in Molecular Biology book series (MIMB, volume 1277)

Abstract

High-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy is a nondestructive technique that is used to obtain the metabolite profile of a tissue sample. This method requires minimal sample preparation. However, it is important to handle the sample with care and keep it frozen during preparation to minimize degradation. Here, we describe a typical protocol for HR-MAS analysis of intact tissue. We also include examples of typical pulse sequence programs and quantification methods that are used today.

Key words

High-resolution magic-angle-spinning NMR spectroscopy Metabolites Tissue analysis Sample storage Sample preparation Sample acquisition Pulse sequences Metabolite quantification 

Notes

Acknowledgments

The authors thank senior engineer Trygve Andreassen for useful consultation regarding MRS acquisition procedures.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Guro F. Giskeødegård
    • 1
    • 2
  • Maria D. Cao
    • 1
    • 2
  • Tone F. Bathen
    • 1
    • 2
    • 3
  1. 1.Department of Circulation and Medical ImagingNorwegian University of Science and Technology (NTNU)TrondheimNorway
  2. 2.St. Olavs HospitalTrondheim University HospitalTrondheimNorway
  3. 3.TrondheimNorway

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