Abstract
Fast separation of DNA and detection of protein/DNA complexes are important in many state-of-the-art molecular medicine technologies, like the production of gene vaccines or medical diagnostics. Here, we describe a nanofluidic chip-based technique for fast, efficient, and virtually label-free detection and separation of protein/DNA and drug/DNA complexes and topological DNA variants. The mechanism is based on a continuous-flow dielectrophoresis at a nanoslit and allows efficient separation of small DNA fragments (<7,000 base pairs) and fast detection of DNA complexes within 1 min.
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Acknowledgment
This work was supported by the German Research Foundation in the collaborative research center (SFB 613) project D2. We thank Anja Rischmüller and Martin Schleef from PlasmidFactory for the production and supply of minicircle DNA.
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Viefhues, M., Regtmeier, J., Anselmetti, D. (2015). Fast and Continuous-Flow Detection and Separation of DNA Complexes and DNA in Nanofluidic Chip Format. In: Van Schepdael, A. (eds) Microchip Capillary Electrophoresis Protocols. Methods in Molecular Biology, vol 1274. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2353-3_9
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DOI: https://doi.org/10.1007/978-1-4939-2353-3_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2352-6
Online ISBN: 978-1-4939-2353-3
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