Sex Identification of Ancient DNA Samples Using a Microfluidic Device

  • Kirsty J. Shaw
  • Keri A. Brown
  • Terence A. Brown
  • Stephen J. Haswell
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1274)

Abstract

Ancient DNA is the name given to the degraded, fragmented, and chemically damaged biomolecules that can be recovered from archaeological remains of plants, animals, and humans. Where ancient human DNA has survived at archaeological sites, it can give valuable information and is especially useful for its potential to identify kinship, population affinities, pathogens, and biological sex. Here, we describe the operation of a microfluidic device for the sex identification of ancient DNA samples using an efficient sample handling process. DNA is extracted from powdered bone samples and abasic sites labeled with biotin. Streptavidin-coated superparamagnetic particles are used to isolate the labeled DNA prior to amplification of the Amelogenin sex marker.

Key words

Ancient DNA DNA extraction Lab-on-a-Chip Polymerase chain reaction Sex identification 

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Copyright information

© Springer Science+Business Media, New York 2015

Authors and Affiliations

  • Kirsty J. Shaw
    • 1
  • Keri A. Brown
    • 2
  • Terence A. Brown
    • 2
  • Stephen J. Haswell
    • 3
  1. 1.Faculty of Science and EngineeringManchester Metropolitan UniversityManchesterUK
  2. 2.Manchester Institute of Biotechnology, Faculty of Life ScienceUniversity of ManchesterManchesterUK
  3. 3.Department of ChemistryUniversity of HullN HumbersideUK

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