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Electrochromatography on Acrylate-Based Monolith in Cyclic Olefin Copolymer Microchip: An Attractive Technology

  • Y. Ladner
  • G. Cretier
  • K. Faure
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1274)

Abstract

Electrochromatography (EC) on a porous monolithic stationary phase prepared within the channels of a microsystem is an attractive alternative for on-chip separation. It combines the separation mechanisms of electrophoresis and liquid chromatography. Moreover, the porous polymer monolithic materials have become popular as stationary phase due to the ease and rapidity of fabrication via free radical photopolymerization. Here, we describe a hexyl acrylate (HA)-based porous monolith which is simultaneously in situ synthesized and anchored to the inner walls of the channel of a cyclic olefin copolymer (COC) device in only 2 min. The baseline separation of a mixture of neurotransmitters including six amino acids and two catecholamines is realized.

Key words

Acrylate-based monolith COC microchip Electrochromatography 

Notes

Acknowledgments

The present process is under French patent no. FR1156586. The authors wish to thank the French National Research Agency for funding project ANR-11-JS09-01701.

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Copyright information

© Springer Science+Business Media, New York 2015

Authors and Affiliations

  1. 1.Institut des Sciences Analytiques, UMR 5280 (CNRS/Université Lyon 1/ENS Lyon)Université de LyonVilleurbanneFrance

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