G Protein-Coupled Receptor Screening Assays pp 189-198

Part of the Methods in Molecular Biology book series (MIMB, volume 1272) | Cite as

Olfactory Receptor Screening Assay Using Nanovesicle-Immobilized Carbon Nanotube Transistor

  • Jong Hyun Lim
  • Juhun Park
  • Seunghun Hong
  • Tai Hyun Park
Protocol

Abstract

Olfactory receptor (OR) genes are considered to be the largest superfamily of the mammalian genome, and in the case of humans, approximately 390 kinds of functional ORs play a role in perceiving odors. In spite of their significance in olfaction, the function of all ORs has not yet been fully revealed. In order to efficiently identify specific ligands of orphan ORs, methods that can generate olfactory signals in a reliable manner and that can convert the cellular signals into measurable responses are required. Here, we describe an OR screening assay method using olfactory sensors that are based on cell-derived nanovesicles combined with single-walled carbon nanotube field-effect transistors (SWNT-FETs). The nanovesicles contain ORs on their surface membrane and induce influx of calcium ions similar to olfactory signal transduction. This ion influx causes an electrical current change along the carbon nanotube, and then this change is measured by the SWNT-FET sensor. This technique facilitates the simple and rapid screening of OR functions.

Key words

Olfactory receptor Olfactory sensor Nanovesicle Carbon nanotube Field-effect transistor 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Jong Hyun Lim
    • 1
  • Juhun Park
    • 2
  • Seunghun Hong
    • 2
    • 3
  • Tai Hyun Park
    • 1
    • 4
  1. 1.School of Chemical and Biological EngineeringSeoul National UniversitySeoulRepublic of Korea
  2. 2.Department of Physics and AstronomySeoul National UniversitySeoulRepublic of Korea
  3. 3.Department of Biophysics and Chemical BiologySeoul National UniversitySeoulRepublic of Korea
  4. 4.Advanced Institutes of Convergence TechnologySuwonRepublic of Korea

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