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Rhodopsin pp 67–76Cite as

Functional Stability of Rhodopsin in a Bicelle System: Evaluating G Protein Activation by Rhodopsin in Bicelles

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1271))

Abstract

Rhodopsin is a prototypical member of the G protein-coupled receptors (GPCRs). This photoreceptor is responsible for initiating the visual signaling transduction cascade upon interaction with its heterotrimeric G protein, transducin (Gt), after light activation. Like all transmembrane proteins, rhodopsin is embedded within a phospholipid bilayer. Many studies have proposed that the membrane composition of this bilayer is an important factor for receptor function during the activation process. Here we describe the methods and assays used to evaluate the function of purified and reconstituted rhodopsin in bicelles.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, Vanderbilt University Medical Center, 442 Robinson Research Building, Nashville, TN, 37232-6600, USA

    Ali I. Kaya, T. M. Iverson & Heidi E. Hamm Ph.D.

  2. Department of Biochemistry, Vanderbilt University Medical Center, Nashville, TN, 37232-6600, USA

    T. M. Iverson

Authors
  1. Ali I. Kaya
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  2. T. M. Iverson
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  3. Heidi E. Hamm Ph.D.
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Corresponding author

Correspondence to Heidi E. Hamm Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA

    Beata Jastrzebska

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Kaya, A.I., Iverson, T.M., Hamm, H.E. (2015). Functional Stability of Rhodopsin in a Bicelle System: Evaluating G Protein Activation by Rhodopsin in Bicelles. In: Jastrzebska, B. (eds) Rhodopsin. Methods in Molecular Biology, vol 1271. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2330-4_5

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  • DOI: https://doi.org/10.1007/978-1-4939-2330-4_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2329-8

  • Online ISBN: 978-1-4939-2330-4

  • eBook Packages: Springer Protocols

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