Rhodopsin pp 363-368 | Cite as

Sustained Delivery of Retinoids to Prevent Photoreceptor Death

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1271)

Abstract

Delivery of hydrophobic compounds to photoreceptors within the retina presents unique challenges due to the anatomy and physiology of the eye. Derivatives of vitamin A (retinoids) are essential to the function and survival of photoreceptors and in the absence of an intrinsic mechanism to metabolize these compounds (visual cycle) leads to extensive loss of photoreceptors and visual function. In this chapter, we describe a method for the sustained delivery of retinoids to young mice that lack a functioning visual cycle to promote survival of photoreceptors.

Key words

Cone death Retina Photoreceptor Retinoid Sustained delivery Visual cycle 

Notes

Acknowledgments

This work was supported by the National Institutes of Health Grants R01 EY04939 (R.K.C.) and CO6 RR015455 (Medical University of South Carolina); Foundation Fighting Blindness, Inc. (R.K.C.); an unrestricted grant (Department of Ophthalmology, Medical University of South Carolina) from Research to Prevent Blindness (RPB); RPB Senior Scientific Investigator Award (R.K.C.); and RPB Medical Student Research Fellowship (P.H.T.).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Ophthalmology and Visual NeurosciencesUniversity of MinnesotaMinneapolisUSA
  2. 2.Department of Ophthalmology, Storm Eye InstituteMedical University of South CarolinaCharlestonUSA

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