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Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus

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Immunocytochemistry and Related Techniques

Part of the book series: Neuromethods ((NM,volume 101))

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Abstract

Adult neurogenesis is the capability of certain brain areas to generate new neurons which integrate into already established neuronal circuits within the adult brain. In most mammals , adult neurogenesis mainly occurs in the olfactory bulb and the hippocampal formation . Adult neurogenesis is thought to consist of several developmental stages that are characterized by morphological distinct cells, and these cells are known to express different markers . These markers and their application for immunohistochemical detection of the specific cell populations are introduced and discussed. Since estimation of the numbers of stained cells is mainly done in brain sections, methods for the quantification of the labeled cells are also highlighted.

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Acknowledgments

Supported by the DFG (BO 1971/5-1; BO 1971/6-1).

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Authors and Affiliations

  1. Institute of Anatomy and Cell Biology, University Medicine Greifswald, Friedrich Loeffler Strasse 23c, 17487, Greifswald, Germany

    Oliver von Bohlen und Halbach

Authors
  1. Oliver von Bohlen und Halbach
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Correspondence to Oliver von Bohlen und Halbach .

Editor information

Editors and Affiliations

  1. Department of Veterinary Sciences, University of Torino, Grugliasco, Torino, Torino, Italy

    Adalberto Merighi

  2. Department of Veterinary Sciences, University of Torino, Grugliasco, Torino, Torino, Italy

    Laura Lossi

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von Bohlen und Halbach, O. (2015). Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus. In: Merighi, A., Lossi, L. (eds) Immunocytochemistry and Related Techniques. Neuromethods, vol 101. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2313-7_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2313-7_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2312-0

  • Online ISBN: 978-1-4939-2313-7

  • eBook Packages: Springer Protocols

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