Abstract
Mitochondria are essential organelles of eukaryotic cells. The vast majority of mitochondrial proteins is encoded within the nuclear genome and translocated into various mitochondrial compartments after translation in the cytosol as preproteins. Even in rather primitive eukaryotes like yeasts, there are 700–1,000 different proteins that need to be recognized in the cytosol, directed to the protein translocases in the two mitochondrial membranes and sorted to their appropriate mitochondrial subcompartment. In vitro reconstituted import systems have proved to be important tools to study these processes in detail. Using isolated mitochondria and radioactively labeled precursor proteins, it was possible to identify several import machineries and pathways consisting of a large number of components during the last few decades.
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Acknowledgments
This work was supported by grants of the Deutsche Forschungsgemeinschaft and the Landesschwerpunkt BioComp of Rheinland-Pfalz.
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Peleh, V., Ramesh, A., Herrmann, J.M. (2015). Import of Proteins into Isolated Yeast Mitochondria. In: Tang, B. (eds) Membrane Trafficking. Methods in Molecular Biology, vol 1270. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2309-0_3
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DOI: https://doi.org/10.1007/978-1-4939-2309-0_3
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