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Methods to Generate Genetically Engineered Mouse Models of Soft Tissue Sarcoma

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Mouse Models of Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1267))

Abstract

We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-KrasG12D/+; p53flox/flox). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer therapies, metastasis, in vivo imaging, and genetic requirements for tumorigenesis.

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Acknowledgments

We thank members of the Kirsch lab for helpful discussions and for contributing to the development of these techniques. We acknowledge members of Amy Wagers lab and Christoph Lepper for developing the satellite cell isolation technique and for graciously sharing their protocols.

Disclosures: None.

Author information

Authors and Affiliations

  1. Duke University Medical Center, Box 91006, Durham, NC, 27708, USA

    Rebecca D. Dodd, Leonor Añó, Jordan M. Blum, Zhizhong Li, David Van Mater & David G. Kirsch

Authors
  1. Rebecca D. Dodd
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  2. Leonor Añó
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  3. Jordan M. Blum
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  4. Zhizhong Li
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  5. David Van Mater
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  6. David G. Kirsch
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Corresponding author

Correspondence to David G. Kirsch .

Editor information

Editors and Affiliations

  1. Medical University Vienna (MUV) Institute for Cancer Research, Vienna, Austria

    Robert Eferl

  2. Research (LBI-CR), Medical University Vienna (MUV), Center of Physiology and Pharmacology & Ludwig Boltzmann Institute for Cancer, Vienna, Austria

    Emilio Casanova

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Dodd, R.D., Añó, L., Blum, J.M., Li, Z., Van Mater, D., Kirsch, D.G. (2015). Methods to Generate Genetically Engineered Mouse Models of Soft Tissue Sarcoma. In: Eferl, R., Casanova, E. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 1267. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2297-0_13

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  • DOI: https://doi.org/10.1007/978-1-4939-2297-0_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2296-3

  • Online ISBN: 978-1-4939-2297-0

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