Abstract
Recent discoveries linking mitochondrial dynamics to various pathologies have generated interest in the design of robust methods to screen proteins involved in mitochondrial dynamics and small molecules that modulate mitochondrial dynamics. Here, we describe functional screening protocols to analyze mitochondrial parameters such as mitochondrial morphology, reactive oxygen species (ROS) levels, mitochondrial calcium, and oxygen consumption rate in cultured cells. Novel proteins that participate in the regulation of mitochondrial dynamics and function can be identified using such an approach.
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Acknowledgments
This study was supported by the MINECO (grant SAF2008-03803), from the “Generalitat de Catalunya” (grant 2009SGR915), CIBERDEM (“Instituto de Salud Carlos III”), and INTERREG IV-B-SUDOE-FEDER (DIOMED, SOE1/P1/E178). A.Z. was the recipient of a Science Intensification Award from the University of Barcelona.
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Muñoz, J.P., Zorzano, A. (2015). Analysis of Mitochondrial Morphology and Function Under Conditions of Mitofusin 2 Deficiency. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1265. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2288-8_21
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DOI: https://doi.org/10.1007/978-1-4939-2288-8_21
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