How FlAsH Got Its Sparkle: Historical Recollections of the Biarsenical-Tetracysteine Tag

  • B. Albert Griffin
  • Stephen R. Adams
  • Roger Y. TsienEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1266)


The biarsenical-tetracysteine tagging system was the first of (and inspiration for) the now numerous methods for site-specifically labeling proteins in living cells with small molecules such as fluorophores. This historical recollection describes its conception and the trial-and-error chemical development required to become a versatile technique.

Key words

FlAsH ReAsH Biarsenical Tetracysteine Protein labeling Genetically targeted 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • B. Albert Griffin
    • 1
  • Stephen R. Adams
    • 2
  • Roger Y. Tsien
    • 2
    • 3
    • 4
    Email author
  1. 1.Independent ResearcherSan DiegoUSA
  2. 2.Department of PharmacologyUniversity of California San DiegoLa JollaUSA
  3. 3.Department of Chemistry and BiochemistryUniversity of California San DiegoLa JollaUSA
  4. 4.Howard Hughes Medical InstituteUniversity of California San DiegoLa JollaUSA

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