Trichomonas vaginalis is a sexually transmitted protozoan parasite of humans. Treatment of trichomoniasis is almost completely dependent on the old drug metronidazole and is hampered by resistance. New drug development, like routine screening for drug resistance, has however been hampered by the lack of reliable screening protocols with sufficient throughput. Here we report on two separate in vitro protocols that use fluorescent dyes and allow for standardized drug sensitivity testing on the required scale.
Trichomonas vaginalisTrichomoniasis Drug screening High-throughput Drug resistance In vitro assay Resazurin Resorufin Propidium iodide Alamar Blue®
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This work was supported through a Research Fellowship from the Government of Saudi Arabia to M.J.N. (grant number S8306) and a Commonwealth scholarship to A.A.E.
Dunne RL et al (2003) Drug resistance in the sexually transmitted protozoan Trichomonas vaginalis. Cell Res 13:239–249PubMedCrossRefGoogle Scholar
Miller MR, Nyirjesy P (2011) Refractory trichomoniasis in HIV-positive and HIV-negative subjects. Curr Infect Dis Rep 13:595–603PubMedCrossRefGoogle Scholar
Diamond LS (1957) The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol 43:488–490PubMedCrossRefGoogle Scholar
Clark CG, Diamond LS (2002) Methods for cultivation of luminal parasitic protists of clinical importance. Clin Microbiol Rev 15:329–341Google Scholar
Giordani RB et al (2009) Anti-Trichomonas vaginalis activity of synthetic lipophilic diamine and amino alcohol derivatives. Biomed Pharmacother 63:613–617PubMedCrossRefGoogle Scholar
Campos Aldrete ME et al (2005) A high-throughput colorimetric and fluorometric microassay for the evaluation of nitroimidazole derivatives anti-Trichomonas activity. Toxicol In Vitro 19:1045–1050PubMedCrossRefGoogle Scholar
Duarte M et al (2009) A quantitative resazurin assay to determinate the viability of Trichomonas vaginalis and the cytotoxicity of organic solvents and surfactant agents. Exp Parasitol 123:195–198PubMedCrossRefGoogle Scholar
Natto MJ et al (2012) Validation of novel fluorescence assays for the routine screening of drug susceptibilities of Trichomonas vaginalis. J Antimicrob Chemother 67:933–943PubMedCrossRefGoogle Scholar