Abstract
The measurement of chronological life span (CLS) in Schizosaccharomyces pombe is traditionally performed by plating back aliquots of aging liquid cultures on solid medium and counting the number of colony-forming units (CFU). However, this method is labor and cost intensive and therefore not amenable to high-throughput screening. Here, we describe a simple method for CLS measurement using aging minicultures in microtiter plates and batch plate-back for the determination of culture viability. This assay can be used to screen a large number of strains, conditions, or compounds in parallel for effects on aging.
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Acknowledgment
This work was supported by the Jürgen Manchot Foundation (to J.S.), the University of Duisburg-Essen, and the Humboldt-Universität zu Berlin.
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Stephan, J., Ehrenhofer-Murray, A.E. (2015). A Method for High-Throughput Analysis of Chronological Aging in Schizosaccharomyces pombe . In: Hempel, J., Williams, C., Hong, C. (eds) Chemical Biology. Methods in Molecular Biology, vol 1263. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2269-7_7
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DOI: https://doi.org/10.1007/978-1-4939-2269-7_7
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2269-7
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