Chemical genomics has the unique potential to expose novel mechanisms of complex cellular biology through screening of small molecules in in vitro assays of a biological phenotype of interest, followed by target identification. In the case of disease-specific assays, the cellular proteins identified might constitute novel drug targets, and the small molecules themselves might be developed as drug leads. In cardiovascular biology, a chemical genomics approach to study the formation of cardiomyocyte, vascular endothelial, and smooth muscle lineages might contribute to therapeutic regeneration. Here, we describe methods used to develop high content screening assays implementing multipotent cardiovascular progenitors derived from human pluripotent stem cells and have identified novel compounds that direct cardiac differentiation.
Human pluripotent stem cells Small molecules Small RNAs High content screening Cardiac differentiation
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This work was supported by grants from the California Institute for Regenerative Medicine (CIRM) (RC00132) and NIH (HL113601, HL108176, and AG043917) to M.M. E.W. was supported by American Heart Association and CIRM training grants.
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