Construction and Application of a Photo-Cross-Linked Chemical Array

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1263)

Abstract

Chemical array technology is a powerful tool for high-throughput screening of small-molecule ligand-protein interactions. A chemical array is a collection of small-molecule compounds spotted and immobilized on a glass slide surface, providing a multiplex platform to identify small-molecule compounds binding to a protein of interest in high-throughput screening. Several research groups have developed a variety of methods for the immobilization of small molecules onto a solid matrix. We have developed a unique photo-cross-linked chemical array for immobilizing small molecules in a functional-group-independent manner. In this chapter, we describe in detail a protocol for the construction of a photo-cross-linked chemical array and its application for ligand screening by using a tag-fused protein.

Key words

Chemical array High-throughput screening Photo-cross-linking Photoaffinity linker Trifluoromethylaryldiazirine PEG Proline Ligand screening 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Yasumitsu Kondoh
    • 1
    • 2
  • Kaori Honda
    • 2
  • Hiroyuki Osada
    • 1
    • 2
  1. 1.Antibiotics LaboratoryRIKENWako, SaitamaJapan
  2. 2.Chemical Biology Research GroupRIKEN Center for Sustainable Resource ScienceWako, SaitamaJapan

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