High-Throughput Small-Molecule Screening in Caenorhabditis elegans

  • Sunitha Rangaraju
  • Gregory M. Solis
  • Michael PetrascheckEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1263)


Chemical compounds, which modulate enzymatic activities or those which induce specific phenotypes of interest, are valuable probes to study biological phenomena, as they allow modulation of enzymatic activities and temporal control of protein action. Here, we describe the methodology to conduct large-scale screens for chemical compounds that induce a desired phenotype in the roundworm Caenorhabditis elegans (C. elegans) using 96- or 384-well microtiter plates.

Key words

Caenorhabditis elegans High-throughput Small molecules Drug screen Chemical libraries 



This work was supported by grant funding to M.P., from the NIH (DP2 OD008398), a grant from the Ellison Medical foundation (AG-NS-0928-12), an MDA Development Grant for S.R., and an NSF GRFP Fellowship for G.M.S. Strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440).


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Sunitha Rangaraju
    • 1
    • 2
    • 3
  • Gregory M. Solis
    • 1
    • 2
    • 3
  • Michael Petrascheck
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Chemical PhysiologyThe Scripps Research InstituteLa JollaUSA
  2. 2.Department of Molecular and Experimental MedicineThe Scripps Research InstituteLa JollaUSA
  3. 3.Department of Molecular and Cellular NeuroscienceThe Scripps Research InstituteLa JollaUSA

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