High-Throughput Small-Molecule Screening in Caenorhabditis elegans
Chemical compounds, which modulate enzymatic activities or those which induce specific phenotypes of interest, are valuable probes to study biological phenomena, as they allow modulation of enzymatic activities and temporal control of protein action. Here, we describe the methodology to conduct large-scale screens for chemical compounds that induce a desired phenotype in the roundworm Caenorhabditis elegans (C. elegans) using 96- or 384-well microtiter plates.
Key wordsCaenorhabditis elegans High-throughput Small molecules Drug screen Chemical libraries
This work was supported by grant funding to M.P., from the NIH (DP2 OD008398), a grant from the Ellison Medical foundation (AG-NS-0928-12), an MDA Development Grant for S.R., and an NSF GRFP Fellowship for G.M.S. Strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440).