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High-Throughput Small-Molecule Screening in Caenorhabditis elegans

  • Sunitha Rangaraju
  • Gregory M. Solis
  • Michael Petrascheck
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1263)

Abstract

Chemical compounds, which modulate enzymatic activities or those which induce specific phenotypes of interest, are valuable probes to study biological phenomena, as they allow modulation of enzymatic activities and temporal control of protein action. Here, we describe the methodology to conduct large-scale screens for chemical compounds that induce a desired phenotype in the roundworm Caenorhabditis elegans (C. elegans) using 96- or 384-well microtiter plates.

Key words

Caenorhabditis elegans High-throughput Small molecules Drug screen Chemical libraries 

Notes

Acknowledgements

This work was supported by grant funding to M.P., from the NIH (DP2 OD008398), a grant from the Ellison Medical foundation (AG-NS-0928-12), an MDA Development Grant for S.R., and an NSF GRFP Fellowship for G.M.S. Strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440).

References

  1. 1.
    Haas J et al (2004) In vivo assay guidelines. In: Sittampalam GS et al (eds) Assay guidance manual. Eli Lilly & Company, Bethesda, MDGoogle Scholar
  2. 2.
    Cabreiro F et al (2013) Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism. Cell 153:228–239PubMedCentralPubMedCrossRefGoogle Scholar
  3. 3.
    Zhang JH, Chung TD, Oldenburg KR (1999) A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4:67–73PubMedCrossRefGoogle Scholar
  4. 4.
    Solis GM, Petrascheck M (2011) Measuring Caenorhabditis elegans life span in 96 well microtiter plates. J Vis Exp 49Google Scholar
  5. 5.
    Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77:71–94PubMedCentralPubMedGoogle Scholar
  6. 6.
    Petrascheck M, Ye X, Buck LB (2007) An antidepressant that extends lifespan in adult Caenorhabditis elegans. Nature 450:553–556PubMedCrossRefGoogle Scholar
  7. 7.
    Petrascheck M, Ye X, Buck LB (2009) A high-throughput screen for chemicals that increase the lifespan of Caenorhabditis elegans. Ann N Y Acad Sci 1170:698–701PubMedCrossRefGoogle Scholar
  8. 8.
    Ye X et al (2014) A pharmacological network for lifespan extension in Caenorhabditis elegans. Aging Cell 13:206–215PubMedCrossRefGoogle Scholar
  9. 9.
    Kwok TC et al (2006) A small-molecule screen in C. elegans yields a new calcium channel antagonist. Nature 441:91–95PubMedCrossRefGoogle Scholar
  10. 10.
    Leiers B et al (2003) A stress-responsive glutathione S-transferase confers resistance to oxidative stress in Caenorhabditis elegans. Free Radic Biol Med 34:1405–1415PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Sunitha Rangaraju
    • 1
    • 2
    • 3
  • Gregory M. Solis
    • 1
    • 2
    • 3
  • Michael Petrascheck
    • 1
    • 2
    • 3
  1. 1.Department of Chemical PhysiologyThe Scripps Research InstituteLa JollaUSA
  2. 2.Department of Molecular and Experimental MedicineThe Scripps Research InstituteLa JollaUSA
  3. 3.Department of Molecular and Cellular NeuroscienceThe Scripps Research InstituteLa JollaUSA

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