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In Vivo Assessment of Mitochondrial Oxygen Consumption

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Mitochondrial Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1264))

Abstract

The protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO2) and mitochondrial oxygen consumption (mitoVO2) in skin. In this work we describe the principles of the method in experimental animals.

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Author information

Authors and Affiliations

  1. Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC – University Medical Center Rotterdam, ‘s-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands

    Floor A. Harms & Egbert G. Mik M.D., Ph.D.

  2. Department of Intensive Care, Erasmus MC – University Medical Center Rotterdam, ‘s-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands

    Egbert G. Mik M.D., Ph.D.

Authors
  1. Floor A. Harms
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  2. Egbert G. Mik M.D., Ph.D.
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Corresponding author

Correspondence to Egbert G. Mik M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Midwestern University, Glendale, Arizona, USA

    Volkmar Weissig

  2. ISANH, Paris, France

    Marvin Edeas

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© 2015 Springer Science+Business Media New York

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Harms, F.A., Mik, E.G. (2015). In Vivo Assessment of Mitochondrial Oxygen Consumption. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_20

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  • DOI: https://doi.org/10.1007/978-1-4939-2257-4_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2256-7

  • Online ISBN: 978-1-4939-2257-4

  • eBook Packages: Springer Protocols

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