Novel Methods for Measuring the Mitochondrial Membrane Potential

  • Roger Springett
Part of the Methods in Molecular Biology book series (MIMB, volume 1264)


The mitochondrial membrane potential is a critical parameter for understanding mitochondrial function, but it is challenging to quantitate with current methodologies which are based on the accumulation of cation indicators. Recently we have introduced a new methodology based on the redox poise of the b-hemes of the bc 1 complex. Here we describe the thermodynamic framework and algorithms necessary to calculate the membrane potential from the measured oxidation states of the b-hemes.

Key words

Mitochondria Membrane potential bc1 complex Redox potential 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of RadiologyDartmouth Medical SchoolHanoverUSA

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