Abstract
High-level expression of recombinant proteins in Escherichia coli often results in accumulation of protein molecules into aggregates known as inclusion bodies (IBs). Isolation of properly folded, bioactive protein from IBs is a cumbersome task and most of the times results in poor recovery. The process of recovering bioactive proteins from IBs consists of solubilization of IB aggregates using denaturants, followed by refolding of the solubilized protein. Here, we describe a simple protocol for screening of buffers for solubilization of IB proteins. Various IB aggregate solubilization methods including organic solvents have been described.
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Acknowledgment
This work is supported by the core grant of the National Institute of Immunology, received from the Dept. of Biotechnology, Govt. of India.
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Singh, A., Upadhyay, V., Panda, A.K. (2015). Solubilization and Refolding of Inclusion Body Proteins. In: GarcÃa-Fruitós, E. (eds) Insoluble Proteins. Methods in Molecular Biology, vol 1258. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2205-5_15
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DOI: https://doi.org/10.1007/978-1-4939-2205-5_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2205-5
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