Abstract
Here, we present a detailed protocol for the detection of activated caspase-8 in axotomized axons of the whole-mounted lamprey spinal cord . This method is based on the use of fluorochrome -labeled inhibitors of caspases (FLICA ) in ex vivo tissue. We offer a very convenient vertebrate model to study the retrograde degeneration of descending pathways after spinal cord injury .
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Acknowledgments
A. Barreiro-Iglesias was supported by a Postdoctoral Fellowship from the Xunta de Galicia. M. I. Shifman was supported by the Shriners Research Foundation (grant number: SHC-85310).
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Barreiro-Iglesias, A., Shifman, M.I. (2015). Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA ). In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_23
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DOI: https://doi.org/10.1007/978-1-4939-2152-2_23
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