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High-Throughput Quantitative Assay for Analyzing Neurite Outgrowth on a Uniform Substratum: The Cell-Substratum Assay

  • Justin A. Beller
  • Thomas M. Hering
  • Diane M. SnowEmail author
Protocol
Part of the Neuromethods book series (NM, volume 93)

Abstract

Neurite outgrowth measurements often require time-consuming measurements of multiple images. The numerous experimental conditions lead to long analysis time and effort. A high-throughput assay provides a quick method to obtain quantitative measures of neurite outgrowth. In this chapter, the experimental setup and imaging protocol are described to efficiently process images of primary neurons or cell lines in order to quantitate parameters.

Key words

Neurite outgrowth Image quantification Software High throughput 

References

  1. 1.
    Condic ML, Snow DM, Letourneau PC (1999) Embryonic neurons adapt to the inhibitory proteoglycan aggrecan by increasing integrin expression. J Neurosci 19(22):10036–10043PubMedGoogle Scholar
  2. 2.
    Snow DM, Lemmon V, Carrino DA, Caplan AI, Silver J (1990) Sulfated proteoglycans in astroglial barriers inhibit neurite outgrowth in vitro. Exp Neurol 109(1):111–130PubMedCrossRefGoogle Scholar
  3. 3.
    Pool M, Thiemann J, Bar-Or A, Fournier AE (2008) NeuriteTracer: a novel ImageJ plugin for automated quantification of neurite outgrowth. J Neurosci Methods 168(1):134–139PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Justin A. Beller
    • 1
  • Thomas M. Hering
    • 1
  • Diane M. Snow
    • 1
    Email author
  1. 1.Spinal Cord and Brain Injury Research Center, Department of Anatomy and NeurobiologyUniversity of KentuckyLexingtonUSA

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