High-Throughput Quantitative Assay for Analyzing Neurite Outgrowth on a Uniform Substratum: The Cell-Substratum Assay

  • Justin A. Beller
  • Thomas M. Hering
  • Diane M. SnowEmail author
Part of the Neuromethods book series (NM, volume 93)


Neurite outgrowth measurements often require time-consuming measurements of multiple images. The numerous experimental conditions lead to long analysis time and effort. A high-throughput assay provides a quick method to obtain quantitative measures of neurite outgrowth. In this chapter, the experimental setup and imaging protocol are described to efficiently process images of primary neurons or cell lines in order to quantitate parameters.

Key words

Neurite outgrowth Image quantification Software High throughput 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Justin A. Beller
    • 1
  • Thomas M. Hering
    • 1
  • Diane M. Snow
    • 1
    Email author
  1. 1.Spinal Cord and Brain Injury Research Center, Department of Anatomy and NeurobiologyUniversity of KentuckyLexingtonUSA

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