Isolation of Perinatal Mouse Cerebral Cortical Astrocytes
Proper functioning of the central nervous system requires collaboration between neurons and glia. Glia are composed of astrocytes, oligodendrocytes, and microglia. In vitro, astrocytes provide support for neuronal cultures for the study of development and regeneration of the brain. Upon injury, astrocytes can produce molecules that protect the harmed area and also prevent further regeneration. Research of astrocyte cell biology and translational uses requires isolation of purified astrocytes. The classical methods for preparing rat astrocytes have been adapted for mouse. In this chapter, we outline a simple method for preparing purified astrocytes from individual perinatal mice.
Key wordsAstrocytes Glial fibrillary acidic protein Vimentin Mouse Glia
- 2.Nicholas CR, Chen J, Tang Y, Southwell DG, Chalmers N, Vogt D, Arnold CM, Chen YJ, Stanley EG, Elefanty AG, Sasai Y, Alvarez-Buylla A, Rubenstein JL, Kriegstein AR (2013) Functional maturation of hPSC-derived forebrain interneurons requires an extended timeline and mimics human neural development. Cell Stem Cell 12:573–586PubMedCentralPubMedCrossRefGoogle Scholar