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Isolation of Perinatal Mouse Cerebral Cortical Astrocytes

  • Rolicia F. Martin
  • Elizabeth M. PowellEmail author
Protocol
Part of the Neuromethods book series (NM, volume 93)

Abstract

Proper functioning of the central nervous system requires collaboration between neurons and glia. Glia are composed of astrocytes, oligodendrocytes, and microglia. In vitro, astrocytes provide support for neuronal cultures for the study of development and regeneration of the brain. Upon injury, astrocytes can produce molecules that protect the harmed area and also prevent further regeneration. Research of astrocyte cell biology and translational uses requires isolation of purified astrocytes. The classical methods for preparing rat astrocytes have been adapted for mouse. In this chapter, we outline a simple method for preparing purified astrocytes from individual perinatal mice.

Key words

Astrocytes Glial fibrillary acidic protein Vimentin Mouse Glia 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Anatomy & Neurobiology, School of MedicineUniversity of MarylandBaltimoreUSA

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