Hydrolytically Degradable Polyethylene Glycol (PEG) Hydrogel: Synthesis, Gel Formation, and Characterization
Degradable tight-mesh hydrogel scaffolds are a promising cell carrier for neural transplantation because they can match the stiffness of the native tissue, decrease immunogenicity of the transplant, and degrade over time as the new tissue forms. Here we describe the synthesis and gel formation of a hydrolytically degradable polyethylene glycol (PEG) hydrogel, where the mild gelation conditions allow for cell encapsulation prior to gel formation. We also describe how to measure swelling ratio and mass loss of the hydrogel over time in order to assess hydrogel degradation.
Key wordsPolyethylene glycol (PEG) Mass Loss Swelling ratio Hydrolytic degradation Azeotropic distillation
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