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Engineered Microdevices to Study and Manipulate Neural Stem Cell Chemotaxis

  • Hui Xu
  • Sarah C. HeilshornEmail author
Protocol
Part of the Neuromethods book series (NM, volume 93)

Abstract

Traditional in vitro chemotaxis tools are typically limited by unstable concentration gradients, presence of hydrodynamic shear, and the inability to decouple cell migration directionality and speed. These limitations have restricted the reproducible and quantitative analysis of neuronal migration, which is a requirement for mechanism-based studies that may guide the development of new therapeutic strategies for neural regeneration. Here we describe a microfluidic setup for creating stable, linear, and shear-free gradients of putative chemotactic cues and their application in quantifying chemotaxis (i.e., migration directionality) and migration speed of human neural progenitor cells (NPCs). These devices can be coated with various extracellular matrix (ECM) proteins for the study of two-dimensional (2D) migration or filled with ECM hydrogels (e.g., collagen I or Matrigel) for the study of three-dimensional (3D) migration.

Key words

Microfluidics Gradient Chemotaxis Migration Neural progenitor cell Extracellular matrix Chemokine Neurotrophic factor 

Notes

Acknowledgment

Work was supported by NIH 1T32-HL098049-01A1 to the Stanford Cardiovascular Institute (H.X.), NIH R21-AR062359-01, and 1DP2-OD006477-01 (S.C.H.).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Materials Science & EngineeringStanford UniversityStanfordUSA

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