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Immunohistochemical Characterization of Brain Neural and Vascular Basement Membranes

  • Zhen HuangEmail author
Protocol
Part of the Neuromethods book series (NM, volume 93)

Abstract

Basement membranes are a critical component of the neural and vascular structures of the brain and play important roles in both brain development and function. Defects in basement membrane integrity are associated with a large number of brain disorders including cobblestone lissencephaly and intracerebral hemorrhage. Here, we describe immunohistochemical approaches for characterizing the neural and vascular basement membranes in the perinatal mouse brain.

Key words

Basement membrane Cerebral cortex Laminin Collagen IV Immunohistochemistry 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of NeurologyUniversity of Wisconsin – MadisonMadisonUSA
  2. 2.Department of NeuroscienceUniversity of Wisconsin – MadisonMadisonUSA

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