Immunohistochemical Characterization of Brain Neural and Vascular Basement Membranes

  • Zhen HuangEmail author
Part of the Neuromethods book series (NM, volume 93)


Basement membranes are a critical component of the neural and vascular structures of the brain and play important roles in both brain development and function. Defects in basement membrane integrity are associated with a large number of brain disorders including cobblestone lissencephaly and intracerebral hemorrhage. Here, we describe immunohistochemical approaches for characterizing the neural and vascular basement membranes in the perinatal mouse brain.

Key words

Basement membrane Cerebral cortex Laminin Collagen IV Immunohistochemistry 


  1. 1.
    Yurchenco PD (2011) Basement membranes: cell scaffoldings and signaling platforms. Cold Spring Harb Perspect Biol 3 (2):pii:a004911. doi: 10.1101/cshperspect.a004911
  2. 2.
    Hohenester E, Yurchenco PD (2012) Laminins in basement membrane assembly. Cell Adh Migr 7(1):56–63PubMedCrossRefGoogle Scholar
  3. 3.
    Yurchenco PD, Ruben GC (1987) Basement membrane structure in situ: evidence for lateral associations in the type IV collagen network. J Cell Biol 105(6 Pt 1):2559–2568PubMedCrossRefGoogle Scholar
  4. 4.
    Cheng YS, Champliaud MF, Burgeson RE, Marinkovich MP, Yurchenco PD (1997) Self-assembly of laminin isoforms. J Biol Chem 272(50):31525–31532PubMedCrossRefGoogle Scholar
  5. 5.
    Barresi R, Campbell KP (2006) Dystroglycan: from biosynthesis to pathogenesis of human disease. J Cell Sci 119(Pt 2):199–207PubMedCrossRefGoogle Scholar
  6. 6.
    Moore SA, Saito F, Chen J, Michele DE, Henry MD, Messing A, Cohn RD, Ross-Barta SE, Westra S, Williamson RA, Hoshi T, Campbell KP (2002) Deletion of brain dystroglycan recapitulates aspects of congenital muscular dystrophy. Nature 418(6896):422–425PubMedCrossRefGoogle Scholar
  7. 7.
    Satz JS, Ostendorf AP, Hou S, Turner A, Kusano H, Lee JC, Turk R, Nguyen H, Ross-Barta SE, Westra S, Hoshi T, Moore SA, Campbell KP (2010) Distinct functions of glial and neuronal dystroglycan in the developing and adult mouse brain. J Neurosci 30(43):14560–14572PubMedCentralPubMedCrossRefGoogle Scholar
  8. 8.
    Graus-Porta D, Blaess S, Senften M, Littlewood-Evans A, Damsky C, Huang Z, Orban P, Klein R, Schittny JC, Muller U (2001) Beta1-class integrins regulate the development of laminae and folia in the cerebral and cerebellar cortex. Neuron 31(3):367–379PubMedCrossRefGoogle Scholar
  9. 9.
    Huang Z, Shimazu K, Woo NH, Zang K, Muller U, Lu B, Reichardt LF (2006) Distinct roles of the beta 1-class integrins at the developing and the mature hippocampal excitatory synapse. J Neurosci 26(43):11208–11219PubMedCentralPubMedCrossRefGoogle Scholar
  10. 10.
    Beggs HE, Schahin-Reed D, Zang K, Goebbels S, Nave KA, Gorski J, Jones KR, Sretavan D, Reichardt LF (2003) FAK deficiency in cells contributing to the basal lamina results in cortical abnormalities resembling congenital muscular dystrophies. Neuron 40(3):501–514PubMedCentralPubMedCrossRefGoogle Scholar
  11. 11.
    Niewmierzycka A, Mills J, St-Arnaud R, Dedhar S, Reichardt LF (2005) Integrin-linked kinase deletion from mouse cortex results in cortical lamination defects resembling cobblestone lissencephaly. J Neurosci 25(30):7022–7031PubMedCentralPubMedCrossRefGoogle Scholar
  12. 12.
    Vahedi K, Alamowitch S (2011) Clinical spectrum of type IV collagen (COL4A1) mutations: a novel genetic multisystem disease. Curr Opin Neurol 24(1):63–68PubMedCrossRefGoogle Scholar
  13. 13.
    Gould DB, Phalan FC, Breedveld GJ, van Mil SE, Smith RS, Schimenti JC, Aguglia U, van der Knaap MS, Heutink P, John SW (2005) Mutations in Col4a1 cause perinatal cerebral hemorrhage and porencephaly. Science 308(5725):1167–1171PubMedCrossRefGoogle Scholar
  14. 14.
    Labelle-Dumais C, Dilworth DJ, Harrington EP, de Leau M, Lyons D, Kabaeva Z, Manzini MC, Dobyns WB, Walsh CA, Michele DE, Gould DB (2011) COL4A1 mutations cause ocular dysgenesis, neuronal localization defects, and myopathy in mice and Walker-Warburg syndrome in humans. PLoS Genet 7(5):e1002062PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of NeurologyUniversity of Wisconsin – MadisonMadisonUSA
  2. 2.Department of NeuroscienceUniversity of Wisconsin – MadisonMadisonUSA

Personalised recommendations