Abstract
Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the living cell using fluorescence cross-correlation spectroscopy.
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Acknowledgments
The author would like to thank Kevin Crosby, Max Tollenaere, Marten Postma, and Ronald Breedijk for their assistance during the experiments. This work was supported by Middelgroot and Echo investment grants from the Netherlands Organisation for Scientific Research (NWO).
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Hink, M.A. (2015). Fluorescence Correlation Spectroscopy. In: Verveer, P. (eds) Advanced Fluorescence Microscopy. Methods in Molecular Biology, vol 1251. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2080-8_8
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DOI: https://doi.org/10.1007/978-1-4939-2080-8_8
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