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Advanced Fluorescence Microscopy pp 263–276Cite as

Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1251))

Abstract

Single-molecule localization-based super-resolution microscopy can be performed with regular, bright, and photostable organic fluorophores. We review a concept termed direct stochastic optical reconstruction microscopy (dSTORM), which operates conventional fluorophores as photoswitches and provides an optical resolution of ~20 nm. We introduce the principle of dSTORM, illustrate experimental schemes, and discuss approaches for data analysis.

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Acknowledgments

The authors thank Marina Dietz, Franziska Fricke, and Bianca Nouvertné for providing super-resolution images. This work was supported by the Bundesministerium für Bildung und Forschung (FORSYS program, grant number 0315262).

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Authors and Affiliations

  1. Institute of Physical & Theoretical Chemistry, Goethe University Frankfurt am Main, Max-von-Laue-Street 7, Frankfurt, 60438, Germany

    Ulrike Endesfelder & Mike Heilemann

Authors
  1. Ulrike Endesfelder
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  2. Mike Heilemann
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Corresponding author

Correspondence to Mike Heilemann .

Editor information

Editors and Affiliations

  1. Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Peter J. Verveer

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Endesfelder, U., Heilemann, M. (2015). Direct Stochastic Optical Reconstruction Microscopy (dSTORM). In: Verveer, P. (eds) Advanced Fluorescence Microscopy. Methods in Molecular Biology, vol 1251. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2080-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-2080-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2079-2

  • Online ISBN: 978-1-4939-2080-8

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