Abstract
An important function of the liver is the synthesis and secretion of blood coagulation factors. Within the liver, hepatocytes are involved in the synthesis of most blood coagulation factors, such as fibrinogen, prothrombin, factor V, VII, IX, X, XI, XII, as well as protein C and S, and antithrombin, whereas liver sinusoidal endothelial cells produce factor VIII and von Willebrand factor. Here, we describe methods for the detection and quantification of most blood coagulation factors in hepatocytes in vitro. Hepatocyte cultures indeed provide a valuable tool to study blood coagulation factors. In addition, the generation and expansion of hepatocytes or hepatocyte-like cells may be used in future for cell-based therapies of liver diseases, including blood coagulation factor deficiencies.
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Acknowledgements
This work was financially supported by the grants of Cosmetics Europe (project HeMiBio, grant agreement No. 266777) and the European Union (FP7) under grant agreement No. 304961 (project Re-Liver).
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Heinz, S., Braspenning, J. (2015). Measurement of Blood Coagulation Factor Synthesis in Cultures of Human Hepatocytes. In: Vinken, M., Rogiers, V. (eds) Protocols in In Vitro Hepatocyte Research. Methods in Molecular Biology, vol 1250. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2074-7_23
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DOI: https://doi.org/10.1007/978-1-4939-2074-7_23
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