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Biological Specimen Collection and Processing for Molecular Analysis

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Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1247))

Abstract

This chapter provides a standardized best practice approach to sample collection and handling for the purpose of nucleic acid (NA) extraction and PCR. These methods are described through text and clearly illustrated figures. Furthermore, for those cases in which transportation of samples cannot be accomplished due to the inability to maintain the cold chain or limitations on the import of live disease agents in diagnostic samples, the chapter describes the use of FTA cards for collection transport and NA preparation of samples for PCR. This chapter also makes emphasis in the best safety practices for handling of samples/tissues to avoid unnecessary disease exposures to laboratory personnel. Finally, NA extraction best practices and methods will be briefly outlined.

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Acknowledgments

Photography credits to Christopher B. Herron, Digital Media Coordinator, College of Veterinary Medicine, The University of Georgia, Athens, USA; art credits to Kip Carter, Chief of Medical Illustration Services, College of Veterinary Medicine, The University of Georgia, Athens, USA.

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Correspondence to Susan Sanchez B.Sc., M.Sc., Ph.D., F.S.B., C.Biol. .

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Sanchez, S., Sellers, H. (2015). Biological Specimen Collection and Processing for Molecular Analysis. In: Cunha, M., Inácio, J. (eds) Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies. Methods in Molecular Biology, vol 1247. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2004-4_5

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  • DOI: https://doi.org/10.1007/978-1-4939-2004-4_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2003-7

  • Online ISBN: 978-1-4939-2004-4

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