Multiple-Locus Variable-Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR and Multicolor Capillary Electrophoresis: Application to the Genotyping of Brucella Species

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1247)

Abstract

The multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) is a genetic typing method based on the evaluation of the number of repeated sequences in multiple selected loci of microbial DNA. Although several MLVA typing panels have been proposed for brucellae, the 16-loci panel is recognized as the standard genotyping method, also used for the Brucella international online database. This chapter describes a high-throughput MLVA-16 protocol using multiplex PCRs and multicolor capillary electrophoresis.

Key words

MLVA Capillary electrophoresis Multiplex PCR Brucella spp Genotyping 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Istituto Zooprofilattico dell’Abruzzo e del Molise “G. Caporale” – National and OIE Reference Laboratory for BrucellosisTeramoItaly

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