In Situ Hybridization with Labeled Probes: Assessment of African Swine Fever Virus in Formalin-Fixed Paraffin-Embedded Tissues
In situ hybridization (ISH) has become a very valuable molecular diagnostic tool to detect specific DNA or RNA sequences in biological samples through the use of complementary DNA- or RNA-labeled probes. Here, we describe an optimized in situ hybridization protocol to detect African swine fever virus (ASFV) DNA in formalin-fixed, paraffin-embedded tissues using digoxigenin-labeled probes.
Key wordsIn situ hybridization Nucleic acid Formalin-fixed paraffin-embedded tissues Digoxigenin Molecular diagnostic tool African swine fever virus
This work has been fully supported by the AGL2010-22229-C03-01 Project from the Ministerio de Economía y Competitividad, Spanish Government.