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In Situ Hybridization with Labeled Probes: Assessment of African Swine Fever Virus in Formalin-Fixed Paraffin-Embedded Tissues

  • Maria Ballester
  • Fernando Rodríguez
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1247)

Abstract

In situ hybridization (ISH) has become a very valuable molecular diagnostic tool to detect specific DNA or RNA sequences in biological samples through the use of complementary DNA- or RNA-labeled probes. Here, we describe an optimized in situ hybridization protocol to detect African swine fever virus (ASFV) DNA in formalin-fixed, paraffin-embedded tissues using digoxigenin-labeled probes.

Key words

In situ hybridization Nucleic acid Formalin-fixed paraffin-embedded tissues Digoxigenin Molecular diagnostic tool African swine fever virus 

Notes

Acknowledgment

This work has been fully supported by the AGL2010-22229-C03-01 Project from the Ministerio de Economía y Competitividad, Spanish Government.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTABellaterraSpain
  2. 2.Centre de Recerca en Agrigenòmica (CRAG), Consorci CSIC-IRTA-UAB-UBBellaterraSpain
  3. 3.Departament de Ciència Animal i dels Aliments, Facultat de VeterinàriaUniversitat Autònoma de BarcelonaBellaterraSpain

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