Abstract
Reverse line blot (RLB) hybridization has become a well-established and widely used method for the multiplex identification of several Babesia and Theileria species in hosts and tick vectors. The procedure is based on the simultaneous PCR amplification of a polymorphic region of the 18S rRNA gene from different piroplasms followed by identification of the individual species by hybridization to species-specific oligonucleotide probes covalently linked to a nylon membrane in a macroarray format.
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Acknowledgments
This work was supported by the Department of Agriculture of the Basque Country Government, the Spanish National Institute for Agricultural and Food Research and Technology (INIA), and the European Regional Development Fund (ERDF).
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Hurtado, A. (2015). Reverse Line Blot Hybridization with Species-Specific Oligonucleotide Probes: Application to Piroplasm Detection. In: Cunha, M., Inácio, J. (eds) Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies. Methods in Molecular Biology, vol 1247. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2004-4_14
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DOI: https://doi.org/10.1007/978-1-4939-2004-4_14
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