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Genotyping Analysis Using an RFLP Assay

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Plant Genotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1245))

Abstract

RFLP (Restriction Fragment Length Polymorphism) is a commonly used technique that can be used for genotyping for nearly all organisms, including plants, animals, and humans. RFLP is widely used in genetic and genomic research, such as genome mapping and gene identification. The technique involves DNA digestion, gel electrophoresis, capillary transfer of DNA, and southern hybridization. In this chapter, we aim to give a detailed introduction of how to perform RFLPs for identifying genotypes.

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Author information

Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China

    Shutao Dai

  2. Institute of Biotechnology, Chinese Academy of Agricultural Science, 12 Zhongguancun South Street, Beijing, 100081, China

    Yan Long

Authors
  1. Shutao Dai
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  2. Yan Long
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Corresponding author

Correspondence to Yan Long .

Editor information

Editors and Affiliations

  1. School of Plant Biology, The University of Western Australia, Crawley, West Australia, Australia

    Jacqueline Batley

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© 2015 Springer Science+Business Media New York

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Dai, S., Long, Y. (2015). Genotyping Analysis Using an RFLP Assay. In: Batley, J. (eds) Plant Genotyping. Methods in Molecular Biology, vol 1245. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1966-6_7

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  • DOI: https://doi.org/10.1007/978-1-4939-1966-6_7

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1965-9

  • Online ISBN: 978-1-4939-1966-6

  • eBook Packages: Springer Protocols

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