Plant Genotyping pp 243-256

Part of the Methods in Molecular Biology book series (MIMB, volume 1245) | Cite as

SNP Genotyping Using KASPar Assays

  • Scott M. Smith
  • Peter J. Maughan
Protocol

Abstract

In a separate chapter we describe a simple method for single nucleotide polymorphism (SNP) discovery using genomic reduction. Here we describe a scalable and cost-effective SNP genotyping method based on KBioscience’s competitive allele-specific PCR amplification of target sequences and endpoint fluorescence genotyping (KASPar™) using a FRET capable plate reader or Fluidigm’s dynamic array high-throughput platform.

Key words

KASPar™ Single nucleotide polymorphism (SNP) Genotyping Fluidigm 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Scott M. Smith
    • 1
  • Peter J. Maughan
    • 2
  1. 1.Department of Plant and Microbial BiologyNorth Carolina State UniversityKannapolisNC
  2. 2.5144 LSB, Department of Plant and Wildlife SciencesBrigham Young UniversityProvoUSA

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