Abstract
We report the development of a simple genomic reduction protocol based on 454-pyrosequencing technology that discovers large numbers of single nucleotide polymorphisms (SNP) from pooled DNA samples. The method is based on the conservation of restriction endonuclease sites across samples and biotin separation for genomic reduction and the addition of multiplex identifier (MID) barcodes to each of the pooled samples to allow for postsequencing deconvolution of the pooled DNA fragments and SNP discovery.
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Acknowledgments
This research was funded by the Ezra Taft Benson Agriculture and Food Institute. We gratefully acknowledge Dr. Edward Wilcox (BYU) for his assistance and advice with regards to 454-pyrosequencing.
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Maughan, P.J., Udall, J.A., Jellen, E.N. (2015). Genomic Reduction Assisted Single Nucleotide Polymorphism Discovery Using 454-Pyrosequencing. In: Batley, J. (eds) Plant Genotyping. Methods in Molecular Biology, vol 1245. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1966-6_13
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DOI: https://doi.org/10.1007/978-1-4939-1966-6_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1965-9
Online ISBN: 978-1-4939-1966-6
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