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Plant Genotyping pp 169–182Cite as

Genomic Reduction Assisted Single Nucleotide Polymorphism Discovery Using 454-Pyrosequencing

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1245))

Abstract

We report the development of a simple genomic reduction protocol based on 454-pyrosequencing technology that discovers large numbers of single nucleotide polymorphisms (SNP) from pooled DNA samples. The method is based on the conservation of restriction endonuclease sites across samples and biotin separation for genomic reduction and the addition of multiplex identifier (MID) barcodes to each of the pooled samples to allow for postsequencing deconvolution of the pooled DNA fragments and SNP discovery.

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Acknowledgments

This research was funded by the Ezra Taft Benson Agriculture and Food Institute. We gratefully acknowledge Dr. Edward Wilcox (BYU) for his assistance and advice with regards to 454-pyrosequencing.

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Authors and Affiliations

  1. 5144 LSB, Department of Plant and Wildlife Sciences, Brigham Young University, 150 East Bulldog Boulevard, Provo, UT, 84602, USA

    Peter J. Maughan

  2. 5133 LSB, Department of Plant and Wildlife Sciences, Brigham Young University, 150 East Bulldog Boulevard, Provo, UT, 84602, USA

    Joshua A. Udall

  3. 4105B LSB, Department of Plant and Wildlife Sciences, Brigham Young University, 150 East Bulldog Boulevard, Provo, UT, 84602, USA

    Eric N. Jellen

Authors
  1. Peter J. Maughan
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  2. Joshua A. Udall
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  3. Eric N. Jellen
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Corresponding author

Correspondence to Peter J. Maughan .

Editor information

Editors and Affiliations

  1. School of Plant Biology, The University of Western Australia, Crawley, West Australia, Australia

    Jacqueline Batley

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Maughan, P.J., Udall, J.A., Jellen, E.N. (2015). Genomic Reduction Assisted Single Nucleotide Polymorphism Discovery Using 454-Pyrosequencing. In: Batley, J. (eds) Plant Genotyping. Methods in Molecular Biology, vol 1245. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1966-6_13

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  • DOI: https://doi.org/10.1007/978-1-4939-1966-6_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1965-9

  • Online ISBN: 978-1-4939-1966-6

  • eBook Packages: Springer Protocols

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