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Application of the High-Resolution Melting Technique for Gene Mapping and SNP Detection in Plants

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Plant Genotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1245))

Abstract

Identifying DNA variations associated with important agronomic traits is a major focus for plant biologists today. Modern crop breeders use molecular markers widely as tools for selecting new varieties more rapidly and efficiently. High-Resolution Melting (HRM) is frequently selected as the method of choice to rapidly and cost effectively detect and genotype SNPs. These SNPs can be used for gene mapping studies and routinely by breeders.

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Acknowledgements

I would like to thank Susan E. Gardiner for useful comments on the manuscript, Roche Applied Science New Zealand for great technical support, and Plant & Food Research for various sources of funding.

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Correspondence to David Chagné .

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Chagné, D. (2015). Application of the High-Resolution Melting Technique for Gene Mapping and SNP Detection in Plants. In: Batley, J. (eds) Plant Genotyping. Methods in Molecular Biology, vol 1245. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1966-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-1966-6_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1965-9

  • Online ISBN: 978-1-4939-1966-6

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