Vacuolar Staining Methods in Plant Cells
Commercially available fluorescent dyes enable the fast and specific visualization of plant vacuoles, allowing for investigation of membrane dynamics and vacuolar biogenesis in living cells. Here, we describe different approaches tinting the tonoplast or the vacuolar lumen with a range of dyes, and illustrate its utilization with established fluorescent-tagged marker lines.
Key wordsTonoplast Vacuole Membrane staining BCECF-AM FM4-64 MDY-64 VAMP711 PIN-FORMED (PIN) Arabidopsis thaliana Bright Yellow-2 (BY-2)
We are grateful to N. Geldner and C. Luschnig for providing material, D. Whittaker for help in preparing the manuscript, and the BOKU-VIBT Imaging Center for access and expertise. This work was supported by the Vienna Science and Technology Fund (WWTF) (to J.K.-V.) and the Deutsche Forschungsgemeinschaft (DFG) (personal postdoctoral research grant to C.L. and D.S.).