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Live Imaging of Root Hairs

  • Silvia M. Velasquez
  • Jose R. DinnenyEmail author
  • José M. EstevezEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 1242)

Abstract

Root hairs are single cells specialized in the absorption of water and nutrients. Growing root hairs requires intensive cell wall changes to accommodate cell expansion at the apical end by a process known as tip growth. The cell wall of plants is a very rigid structure comprised largely of polysaccharides and hydroxyproline-rich O-glycoproteins. The importance of root hairs stems from their capacity to expand the surface of interaction between the root and the environment, in search for the necessary nutrients and water to allow plant growth. Therefore, it becomes crucial to deepen our knowledge of them, particularly in the light of the applicability in agriculture by allowing the expansion of croplands. Root hair growth is an extremely fast process, reaching growth rates of up to 1 μm/min and it also is a dynamic process; there can be situations in which the final length might not be affected but the growth rate is. Consequently, in this chapter we focus on a method for studying growth dynamics and rates during a time course. This method is versatile allowing for it to be used in other plant organs such as lateral root, hypocotyl, etc., and also in various conditions.

Key words

Roots hairs Tip growth Growth rate Arabidopsis 

Notes

Acknowledgement

This work was supported by PICT2011-0054 (J.M.E.), Fulbright Fellowship (S.M.V.), and Mizutani Grant (J.M.E.).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresBuenos AiresArgentina
  2. 2.Department of Plant BiologyCarnegie Institution for ScienceStanfordUSA
  3. 3.Laboratorio de Fisiología y Biología Molecular, IFIByNE (CONICET), FCEyNUniversidad de Buenos AiresBuenos AiresArgentina

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